Figure 6.
In THP-1 monocytes, but not in Calu-3 cells, VEA 54% (3.7 and 5 V) induced a significant and dose-dependent reactive oxygen species (ROS) generation. Acute in vitro assessment of ROS generation in human lung epithelial cells (Calu-3) (A, C) and peripheral monocytes (THP-1) (B, D) exposed for 24 h to aerosols generated from different concentrations of Δ8THC/VEA or nicotine-based e-liquids under 2 operational voltages (3.7 or 5 V), or cigarette smoke, at 2 doses of 85 and 257 puffs/m2 (1 and 3 months). All values are represented as the mean± SE. * p < .05, ** p < .01, *** p < .001, **** p < .0001 compared with negative control. # p < .05, ## p < .01, ### p < .001, #### p < .0001 compare between 2 doses. Statistical analysis was performed on an average of 3 independent biological samples; fold changes >20% were deemed to be statistically significant (p < .05).