Figure 1.

Morphology of RPEs or cardiomyocytes derived from iPSC clones and their transplants before transplantation. (A) iPSCs 16E84, 16E85, 16H12, and 15M38 or H9 ESCs (control) were cultured using an RPE differentiation protocol. Cells differentiated into RPEs were termed iPSC-RPEs or H9-RPEs (upper panels) and cells other than RPEs were termed iPSC-non RPEs or H9-non RPEs (lower panels). RPEs or non RPEs derived from iPSCs prior to transplantation and relevant transplantation after 5 months of monitoring are shown in upper and lower panels, respectively. The differentiation efficiency to RPEs in the whole culture dish is indicated as a percentage and appended in the photos of RPEs. (B) iPSCs 16E84, 16E85, 16H12, and 15M38 or H9 ESCs (control) were cultured with a cardiomyocyte (CM) protocol. Cells differentiated into CMs (red dotted line) were termed iPSC-CMs or H9-CMs (upper panels) and cells other than CMs (White dotted line) were termed iPSC-non CMs or H9-non CMs (lower panels). CMs or non CMs derived from iPSCs prior to transplantation and relevant transplantation after 5 months of monitoring are shown in upper and lower panels, respectively. The differentiation efficiency to CMs in whole culture dishes was assessed by immunostaining with cardiac troponin T and is shown as a percentage appended in the photographs in the upper panels.