Appendix 1—key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | MYLK2 Antibody, rabbit polyclonal |
Invitrogen | Cat#PA5-29324 RRID: AB_2546800 |
(1:1000) |
| Antibody | Total OXPHOS Human WB Antibody Cocktail, mouse monoclonal | Abcam | Cat#ab110411 RRID: AB_2756818 |
(1:1000) |
| Antibody | Sirt1(Sir2) Antibody, rabbit polyclonal | Millipore Sigma | Cat#07–131 RRID: AB_10067921 |
(1:1000) |
| Antibody | Sirt3 (D22A3) Antibody, rabbit monoclonal | Cell Signaling Technologies | Cat#5490 RRID: AB_10828246 |
(1:2000) |
| Antibody | Sirt5 Antibody, rabbit monoclonal | Cell Signaling Technologies | Cat#8782 RRID: AB_2716763 |
(1:1000) |
| Antibody | Sirt6 Antibody, rabbit monoclonal | Cell Signaling Technologies | Cat#12486 RRID: AB_2636969 |
(1:1000) |
| Antibody | Acetyl-lysine Antibody, rabbit polyclonal | Cell Signaling Technologies | Cat#9441 RRID: AB_331805 |
(1:1000) |
| Antibody | Ac-SOD2 K68 Antibody, rabbit monoclonal | Abcam | Cat#ab13737 RRID: AB_2784527 |
(1:1000) |
| Antibody | Ac-SOD2 K122 Antibody, rabbit monoclonal | Abcam | Cat#ab214675 RRID: AB_2892634 |
(1:1000) |
| Antibody | P300 Mouse Antibody, mouse monoclonal | Santa Cruz Biotechnologies | Cat#sc48343 RRID: AB_628075 |
(1:200) |
| Antibody | P300 Mouse Antibody, mouse monoclonal | Santa Cruz Biotechnologies | Cat#sc32244 RRID: AB_628076 |
(1:500) |
| Antibody | Ac-H2B K20 Antibody, rabbit monoclonal | Abcam | Cat#ab177430 | (1:1000) |
| Antibody | H2B Antibody, rabbit monoclonal | Cell Signaling Technologies | Cat#12364 RRID: AB_2714167 |
(1:3000) |
| Antibody | Ac-H3 K23 Antibody, rabbit monoclonal | Cell Signaling Technologies | Cat#14932 RRID: AB_2798650 |
(1:1000) |
| Antibody | H3 Antibody, rabbit monoclonal | Cell Signaling Technologies | Cat#4499 RRID: AB_10544537 |
(1:3000) |
| Antibody | Goat Anti-Rabbit Ig, Human ads-HRP, goat polyclonal | Southern Biotech | Cat#4010–05 RRID: AB_2632593 |
(1:5000) |
| Antibody | Goat Anti-Mouse, goat polyclonal | Dako (Agilent) | Cat# P0447 RRID: AB_2617137 |
(1:5000) |
| Biological sample (human) | Human skeletal muscle (vastus lateralis) | This paper | Not externally available | |
| Cell line (mouse) | C2C12 (ATCC CRL-1772) | ATCC | Cat#1722 RRID: CVCL_0188 |
Authenticated by ATCC – CO1 assay. Tested in-house for mycoplasma – negative. |
| Chemical compound, drug | Glycerol | Millipore Sigma | Cat# G5516; CAS# 56-81-5 | |
| Chemical compound, drug | Sodium pyrophosphate decahydrate | Millipore Sigma | Cat# 221368; CAS# 13472-36-1 | |
| Chemical compound, drug | Sodium chloride | Millipore Sigma | Cat# S9888; CAS# 7647-14-5 | |
| Chemical compound, drug | HEPES | Millipore Sigma | Cat# H3375; CAS# 7365-45-9 | |
| Chemical compound, drug | IGEPAL CA-630, NP-40 | Millipore Sigma | Cat# I8896; CAS# 9002-93-1 | |
| Chemical compound, drug | β-Glycerophosphate disodium salt hydrate | Millipore Sigma | Cat# G9422; CAS# 154804-51-0 | |
| Chemical compound, drug | Sodium orthovanadate | Millipore Sigma | Cat# S6508; CAS# 13721-39-6 | |
| Chemical compound, drug | Sodium fluoride | Millipore Sigma | Cat# S1504; CAS# 7681-49-4 | |
| Chemical compound, drug | Phenylmethanesulfonyl fluoride | Millipore Sigma | Cat# P7626; CAS# 329-98-6 | |
| Chemical compound, drug | EDTA (for electrophoresis) | Millipore Sigma | Cat# E5134; CAS# 6381-92-6 | |
| Chemical compound, drug | EGTA | Millipore Sigma | Cat# E4378; CAS# 67-42-5 | |
| Chemical compound, drug | Aprotinin from bovine lung | Millipore Sigma | Cat# A1153; CAS# 9087-70-1 | |
| Chemical compound, drug | Leupeptin | Millipore Sigma | Cat# L2884; CAS# 103476-89-7 | |
| Chemical compound, drug | Benzamidine hydrochloride hydrate | Millipore Sigma | Cat# B6506; CAS# 206752-36-5 | |
| Chemical compound, drug | Bovine serum albumin | Millipore Sigma | Cat# A7906; CAS# 9048-46-8 | |
| Chemical compound, drug | Trizma base | Millipore Sigma | Cat# T1503; CAS# 77-86-1 | |
| Chemical compound, drug | DL-Dithiothreitol | Millipore Sigma | Cat# D0632 and 43819; CAS# 3483-12-3 | |
| Chemical compound, drug | Sodium dodecyl sulfate | Millipore Sigma | Cat# L3771; CAS# 151-21-3 | |
| Chemical compound, drug | Bromophenol Blue sodium salt | Millipore Sigma | Cat# B8026; CAS# 34725-61-6 | |
| Chemical compound, drug | Tween 20 | Millipore Sigma | Cat# P9416; CAS# 9005-64-5 | |
| Chemical compound, drug | Skimmed milk powder | Easis | Cat# 801,300 | |
| Chemical compound, drug | Glycine | Millipore Sigma | Cat# G7126; CAS# 56-40-6 | |
| Chemical compound, drug | 2-propanol | Millipore Sigma | Cat# 109634; CAS# 67-63-0 | |
| Chemical compound, drug | Ethanol 96% | Plum | Cat# 201146; CAS# 64-17-5 | |
| Chemical compound, drug | Brilliant Blue R | Millipore Sigma | Cat# B7920; CAS# 6104-59-2 | |
| Chemical compound, drug | Acetic acid (glacial) 100% | Millipore Sigma | Cat# 100063; CAS# 64-19-7 | |
| Chemical compound, drug | Precision plus all blue | BioRad | Cat# 1610373 | |
| Chemical compound, drug | Precision plus dual color | BioRad | Cat# 1610374 | |
| Chemical compound, drug | Immobilon Forte Western HRP substrate | Millipore Sigma | Cat# WBLUF 0500 | |
| Chemical compound, drug | Bovine Serum Albumin Standard | Thermo Fisher Scientific | Cat# 23,209 | |
| Chemical compound, drug | BCA Protein Assay Reagent A | Thermo Fisher Scientific | Cat# 23,223 | |
| Chemical compound, drug | BCA Protein Assay Reagent B | Thermo Fisher Scientific | Cat# 23,224 | |
| Chemical compound, drug | Potassium phosphate dibasic trihydrate | Millipore Sigma | Cat# P9666; CAS# 16788-57-1 | |
| Chemical compound, drug | Potassium dihydrogen phosphate | Millipore Sigma | Cat# 1.04873; CAS# 7778-77-0 | |
| Chemical compound, drug | BCA Protein Assay Kit | Thermo Fisher Scientific | Cat# 23,225 | |
| Chemical compound, drug | Trizma hydrochloride | Millipore Sigma | Cat# T3253; CAS# 1185-53-1 | |
| Chemical compound, drug | EDTA | Millipore Sigma | Cat# E9884; CAS# 60-00-4 | |
| Chemical compound, drug | NAD | Millipore Sigma | Cat# NAD100-RO; CAS# 53-84-9 | |
| Chemical compound, drug | L-(−)-Malic acid sodium salt | Millipore Sigma | Cat# M1125; CAS# 68303-40-2 | |
| Chemical compound, drug | Acetyl-Coenzyme A | Millipore Sigma | Cat# ACOA-RO | |
| Chemical compound, drug | L-Malate Dehydrogenase (L-MDH) | Millipore Sigma | Cat# LMDH-RO | |
| Chemical compound, drug | NADH | Millipore Sigma | Cat# 10107735001 | |
| Chemical compound, drug | Sucrose | Millipore Sigma | Cat# S7903; CAS# 57-50-1 | |
| Chemical compound, drug | Magnesium chloride hexahydrate | Millipore Sigma | Cat# M2670; CAS# 7791-18-6 | |
| Chemical compound, drug | Taurine | Millipore Sigma | Cat# T0625; CAS# 107-35-7 | |
| Chemical compound, drug | Potassium Dihydrogen Phosphate | Millipore Sigma | Cat# P9791; CAS# 7778-77-0 | |
| Chemical compound, drug | HEPES | Millipore Sigma | Cat# H3375; CAS# 7365-45-9 | |
| Chemical compound, drug | Bovine serum albumin (fatty acid free) | Millipore Sigma | Cat# A7030; CAS# 9048-46-8 | |
| Chemical compound, drug | Catalase from bovine liver | Millipore Sigma | Cat# C9322; CAS# 9001-05-2 | |
| Chemical compound, drug | Lactobionic acid | Millipore Sigma | Cat# 153516; CAS# 96-82-2 | |
| Chemical compound, drug | MES potassium salt | Millipore Sigma | Cat# M0895; CAS# 39946-25-3 | |
| Chemical compound, drug | ATP | Millipore Sigma | Cat# A3377; CAS# 34369-07-8 | |
| Chemical compound, drug | Sodium creatine phosphate dibasic tetrahydrate | Millipore Sigma | Cat# 27920; CAS# 71519-72-7 | |
| Chemical compound, drug | Imidazole | Millipore Sigma | Cat# I0250; CAS# 288-32-4 | |
| Chemical compound, drug | Potassium hydroxide | Millipore Sigma | Cat# P1767; CAS# 1310-58-3 | |
| Chemical compound, drug | Calcium carbonate | Millipore Sigma | Cat# C4830; CAS# 471-34-1 | |
| Chemical compound, drug | Saponin from quillaja bark | Millipore Sigma | Cat# S7900; CAS# 8047-15-2 | |
| Chemical compound, drug | L-(−)-Malic acid sodium salt | Millipore Sigma | Cat# M1125; CAS# 68303-40-2 | |
| Chemical compound, drug | Octanoyl-L-carnitine | Millipore Sigma | Cat# 50892; CAS# 25243-95-2 | |
| Chemical compound, drug | ADP | Millipore Sigma | Cat# A5285; CAS# 72696-48-1 | |
| Chemical compound, drug | L-glutamic acid monosodium salt monohydrate | Millipore Sigma | Cat# 49621; CAS# 6106-04-3 | |
| Chemical compound, drug | Sodium succinate dibasic hexahydrate | Millipore Sigma | Cat# S2378; CAS# 6106-21-4 | |
| Chemical compound, drug | Cytochrome c from equine heart | Millipore Sigma | Cat# C2506; CAS# 9007-43-6 | |
| Chemical compound, drug | FCCP | Millipore Sigma | Cat# C2920; CAS# 370-86-5 | |
| Chemical compound, drug | Rotenone | Millipore Sigma | Cat# R8875; CAS# 83-79-4 | |
| Chemical compound, drug | Malonic acid | Millipore Sigma | Cat# M1296; CAS# 141-82-2 | |
| Chemical compound, drug | Myxothiazol | Millipore Sigma | Cat# T5580; CAS# 76706-55-3 | |
| Chemical compound, drug | Antimycin A from Streptomyces sp. | Millipore Sigma | Cat# A8674; CAS# 1397-94-0 | |
| Chemical compound, drug | Sodium deoxycholate | Sigma-Aldrich | Cat# D6750 CAS# 302-95-4 |
|
| Chemical compound, drug | Tris(2-carboxyethyl)phosphine hydrochloride | Sigma-Aldrich | Cat# C4706 CAS# 51805-45-9 |
|
| Chemical compound, drug | 2-Chloroacetamide | Sigma-Aldrich | Cat# 22,790 CAS# 79-07-2 |
|
| Chemical compound, drug | Trypsin | Sigma-Aldrich | Cat# T7575 | |
| Chemical compound, drug | Lysine | Wako | Cat# 124–06871 | |
| Chemical compound, drug | DMEM high glucose | Gibco | Cat# 41965039 | |
| Chemical compound, drug | Fetal bovine serum(FBS) | Sigma-Aldrich | Cat# F7524 | |
| Chemical compound, drug | Penicillin-Streptomycin, liquid | Gibco | Cat# 15070–063 | |
| Chemical compound, drug | Opti-MEM I Reduced Serum Medium | Gibco | Cat# 31985062 | |
| Chemical compound, drug | TransIT-X2 Dynamic Delivery System | Mirus Bio | Cat# MIR6003 | |
| Sequence-based reagent | EP300 siRNA | Merck Sigma | Cat# SASI_Mm01_00159721 | |
| Commercial assay or kit | PTMScan Acetyl-Lysine Motif [Ac-K] Kit | Cell Signaling Technologies | Cat#13,416 | |
| Software, algorithm, | Graphpad Prism 8.0 | https://www.graphpad.com/ | RRID: SCR_002798 | |
| Software, algorithm | MaxQuant 1.5.3.30 | https://maxquant.org/ | RRID: SCR_014485 | |
| Software, algorithm | Spectronaut v14 | https://biognosys.com/shop/spectronaut | ||
| Software, algorithm | Perseus 1.6.10.50 | http://www.coxdocs.org/doku.php?id=perseus:start | RRID: SCR_015753 | |
| Software, algorithm | R Studio | https://rstudio.com/ | RRID: SCR_000432 | |
| Software, algorithm | Cytoscape v3.7.2 | https://www.cytoscape.org | RRID: SCR_003032 | |
| Software, algorithm | iceLogo | https://iomics.ugent.be/icelogoserver/ | RRID: SCR_012137 | |
| Software, algorithm | Adobe Illustrator 24.3 | https://www.adobe.com/products/illustrator | RRID: SCR_010279 | |
| Software, algorithm | Bio-Rad Image Studio | https://www.licor.com/bio/products/Software, algorithm/image_studio/ | RRID: SCR_014210 | |
| Software, algorithm | Oroboros DatLab 6 V. 6.1.0.7 | https://www.oroboros.at/index.php/product/datlab/ | ||
| Other | Stainless Steel Beads 5 mm | Qiagen | Cat# 69989 | Lysis reagent |
| Other | Stuart Rotator Drive STR4 | Stuart-equipment | Cat# STR4 | Lysis reagent |
| Other | Hettich universal 320 R centrifuge | Andrea Hettich GmbH, Germany | Cat# 1406 | Lysis reagent |
| Other | Emax precision microplate reader | Molecular Devices | Cat# LR88026 | Protein concentration quantification |
| Other | White 96-Well Immuno Plates | Fischer Scientific | Cat# 10415985 | Protein concentration quantification |
| Other | Fluoroskan, Microplate Fluorometer, one dispenser | Fischer Scientific | Cat# 5200111 | Protein concentration quantification |
| Other | Multiskan FC Microplate Photometer | Fischer Scientific | Cat# 11500695 | Protein concentration quantification |
| Other | PowerPac HC Power Supply | BioRad | Cat# 1645052 | Immunoblotting reagent |
| Other | TE 77 ECL Semi-Dry Transfer Unit | Amersham Biosciences | Cat# 80-6211-86 | Immunoblotting reagent |
| Other | ChemiDoc MP Imaging System | BioRad | Cat# 731BR00119 | Immunoblotting reagent |
| Other | 10% Criterion TGX Stain-Free gel | BioRad | Cat# 567–8035 | Immunoblotting reagent |
| Other | 16.5% Criterion Tris-Tricine/Peptide gel | BioRad | Cat# 345–0065 | Immunoblotting reagent |
| Other | Immobilon-P PVDF membrane | Millipore Sigma | Cat# IPVH00010 | Immunoblotting reagent |
| Other | Whatman cellulose chromatography papers | Millipore Sigma | Cat# WHA3030917 | Immunoblotting reagent |
| Other | Sep-Pak C18 Cartrige | Waters | Cat# WAT054955 | Proteomic sample preparation |
| Other | Non-treated 96-Well Microplates | Fischer Scientific | Cat# 10252711 | Proteomic sample preparation |