Fig. 2.

Relative expression level of lncR17454 (A and B), let-7 (C and D), miR-100 (E and F), and miR-2795 (G and H) at different developmental stages of silkworm. La1-2d, La2-2d, La3-2d, and La4-2d represent the 2-d-old first-, second-, third-, and fourth-instar larvae, respectively. La5-3d represented the 3-d-old fifth-instar larvae. Pre-P-1d and Pre-P-2d represented the first and second day of pre-pupa, respectively. P-1d, P-5d represented the first day and the fifth day of pupa. A-1d means the virgin moth. actinA3 and U6 were used as the internal control of lncRNA and miRNAs, respectively. Three independent experiments were conducted and each sample had three repeats. Significance analysis was conducted with ANOVA and Student’s t-test.