Table 6.
The effect of maternal PRRSV infection and dietary supplementation with soy isoflavones on offspring proportional lymphocyte populations
| DPV 0 | DPV 7 | DPV 14 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Item | CON | POS | ISF | SEM | CON | POS | ISF | SEM | CON | POS | ISF | SEM | Treatment P-value | Time P-value | Treatment × Time P-value |
| Pigs1, n | 16 | 15 | 12 | 16 | 15 | 12 | 16 | 15 | 12 | ||||||
| Lymphocytes2, n | 5654 | 5021 | 4281 | 719 | 4881 | 5055 | 5004 | 738 | 6022 | 5065 | 4605 | 720 | 0.61 | 0.56 | 0.12 |
| Lymphocytes3, % | 56.54 | 50.21 | 42.81 | 7.20 | 48.81 | 50.55 | 50.04 | 7.38 | 60.22 | 50.65 | 46.05 | 7.20 | 0.61 | 0.56 | 0.12 |
| Total T cells4, % | 64.82xy | 65.77x | 53.75 | 5.10 | 61.77x | 66.55xy | 55.21 | 5.20 | 66.9y | 71.76y | 56.07 | 5.10 | 0.18 | 0.03 | 0.54 |
| Undifferentiated T cells5, % | 53.47xy | 52.05x | 41.27x | 4.51 | 49.06x | 51.95x | 41.12x | 4.63 | 56.73y | 61.06y | 47.61y | 4.51 | 0.15 | < 0.01 | 0.56 |
| Helper T cells6, % | 21.12x | 18.29x | 18.40x | 1.34 | 17.96y | 17.68x | 18.43x | 1.42 | 12.57z | 12.15y | 15.52y | 1.34 | 0.60 | < 0.01 | 0.02 |
| Cytotoxic T cells7, % | 21.24 | 22.78 | 29.28 | 3.42 | 25.95 | 21.85 | 29.91 | 3.53 | 25.03 | 19.77 | 28.91 | 3.42 | 0.22 | 0.51 | 0.25 |
| Dual Positive T cells8, % | 4.18ax | 6.79ab | 11.05bx | 1.80 | 7.03y | 8.27 | 10.12x | 1.84 | 5.72xy | 6.88 | 7.96y | 1.80 | 0.25 | 0.02 | 0.02 |
| Helper:Cytotoxic T cells | 1.12x | 0.92x | 0.75 | 0.16 | 0.80y | 0.86x | 0.66 | 0.16 | 0.60z | 0.66y | 0.57 | 0.16 | 0.64 | < 0.01 | 0.06 |
Least squares means within a day across treatments lacking a common superscript letter differ (P < 0.05).
Least squares means within a treatment across days lacking a common superscript letter differ (P < 0.05).
Data were analyzed using a split-plot design, with litter serving as whole plot and offspring pig serving as split plot.
Absolute count of lymphocytes detected.
Lymphocytes, % = (absolute count of lymphocytes/10,000 [total number of cells counted per sample]) × 100.
Total T cells, % = (Number of CD3+ cells/Number of lymphocytes) × 100.
Undifferentiated T cells, % = 100 − (Helper T cells, % + Cytotoxic T cells, % + Dual Positive T cells, %).
Helper T cells defined as CD3+ CD4+ cells.
Cytotoxic T cells defined as CD3+ CD8+ cells.
Dual Positive T cells defined as CD3+CD4+CD8+ cells.
Abbreviations: CON, control treatment (unsupplemented diet without infection); DPV, days post-vaccination; ISF, isoflavone-supplemented treatment (with infection); POS, positive control (unsupplemented diet with infection); SEM, standard error of the mean.