. Author manuscript; available in PMC: 2022 May 31.

Published in final edited form as: J Vis Exp. 2022 Apr 7;(182):10.3791/63539. doi: 10.3791/63539

Table 2:

Echocardiographic evaluation of coronary artery flow in 7-day old mouse pups.

Coronary Flow Parameters		Baseline	5 min	CFR
Coronary Flow Parameters		Isoflurane 1.5%	Isoflurane 2.5%	5 min/baseline
Diastole	Peak velocity (mm/s)	516.58 ± 113.04	599.43 ± 101.34	1.18 ± 0.18
	Mean velocity (mm/s)	308.50 ± 63.44	351.50 ± 53.98
	VTI (mm)	25.23 ± 5.86	30.65 ± 7.75
Sytole	Peak velocity (mm/s)	121.81 ± 40.52	163.13 ± 32.59^*
	Mean velocity (mm/s)	84.82 ± 27.16	114.70 ± 21.84^*
	VTI (mm)	5.21 ± 1.84	7.76 ± 2.08^*
	Heart rate (bpm)	536.20 ± 128.90	540.80 ± 233.15
	Respiratory rate (rpm)	69.60 ± 15.89	38.80 ± 24.18

Seven-day old mice, N = 7; data presented as mean ± SD; the Student’s t-test was used to analyze the data;

p < 0.05; CFR = coronary flow reserve; VTI = velocity time integral.