FIG. 1.
Agarose gel (1%) electrophoresis of PCR products obtained with universal and specific primers for cry9 genes. Lanes 1 and 8, molecular weight markers (λDNA cleaved by HindIII), with sizes (in kilobases) indicated on left; lanes 2 to 4, respectively, DNA of field-collected isolates U-27, K-74, and B. thuringiensis subsp. aizawai HD-133 amplified with Un9; lanes 5 to 7, respectively, DNA of field-collected isolates U-27, K-74, and B. thuringiensis subsp. aizawai HD-133 amplified with a mixture of four specific primers and one reverse Un9(r).