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. 2022 May 13;6(7):bvac078. doi: 10.1210/jendso/bvac078

Figure 2.

Figure 2.

ZNF217 expression in normal and polycystic ovary syndrome (PCOS) theca cells. A, ZNF217 (green signal) is localized in punctate nuclear structures in human theca cells by immunofluorescence. The ZNF217 signal is reduced in PCOS theca cells compared to normal theca cells cultured without forskolin, and in normal theca cells treated with forskolin (20 μM, 24 hours). B, Representative Western blots comparing ZNF217 and DENND1A.V2 protein expression in 2 normal and 2 PCOS theca cell preparations cultured in the C, absence, or F, presence of forskolin (20 μM) for 24 hours. C, Quantitative Western blot data from 5 normal and 5 PCOS preparations demonstrated that ZNF217 protein was decreased by forskolin treatment in normal theca cells (αP < .001). ZNF217 protein in PCOS theca cells was decreased compared to normal theca cells (***P < .001) under control culture conditions. D, Relative DENND1A.V2 protein normalized by mammalian target of rapamycin (mTOR) was elevated both in forskolin-treated (***P < .005) and untreated (***P < .005) PCOS cells.