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. 2022 May 13;6(7):bvac078. doi: 10.1210/jendso/bvac078

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© The Author(s) 2022. Published by Oxford University Press on behalf of the Endocrine Society.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — ZNF217 knockdown in normal theca cells results in augmented DENND1A.V2 and CYP17A1 messenger RNA (mRNA) expression. Normal theca cells (N=5) were infected with equivalent amounts of control short hairpin RNA (shRNA) or ZNF217 shRNA lentiviral particles (3 pmol/10⁶ cells) for 24 hours, then treated for 36 hours in the absence (control) or presence of 20 μM forskolin. A, In the absence of forskolin, ZNF217 expression was decreased following infection with ZNF217 lentiviral shRNA (*P < .05) as compared control shRNA. Forskolin treatment inhibited ZNF217 mRNA in control shRNA-infected cells (^αP < .05). B, DENND1A.V2 expression was increased following ZNF217 shRNA lentivirus infection under both control (***P < .001) and forskolin-stimulated (***P < .001) conditions. C, CYP17 A1 mRNA was increased by forskolin-stimulation in normal theca cells infected with control shRNA (^αP < .01). CYP17 A1 mRNA was increased following ZNF217 shRNA lentivirus infection under both control (*P < .01) and forskolin-stimulated (**P < .001) conditions, compared to control shRNA-infected cells.