Table 1.
Primers used in this study
| Amplified product | Primer pair | Sequence (5′ to 3′) | Amplicon size (bp) | Annealing temperature (°C) | Reference |
|---|---|---|---|---|---|
| gyrA | gyrA-F | AGTCCTATCTCGACTACGCGAT | 341 | 55 | 17 |
| gyrA-R | AGTCGACGGTTTCCTTTTCCAG | ||||
| gyrB | gyrB-F | TGCGGTGGAACAGGAGATGGGCAAGTAC | 697 | 55 | 17 |
| gyrB-R | CTGGCGGAAGAAGAAGGTCAACAGCAGGGT | ||||
| parC | parC-F | CGAGCAGGCCTATCTGAACTAT | 235 | 55 | 17 |
| parC-R | GAAGGACTTGGGATCGTCCGGA | ||||
| parE | parE-F | CGGCGTTCGTCTCGGGCGTGGTGAAGGA | 592 | 65 | 4 |
| parE-R | TCGAGGGCGTAGTAGATGTCCTTGCCGA | ||||
| nalC | nalC-F | TCAACCCTAACGAGAAACGCT | 814 | 69 | 4 |
| nalC-R | TCCACCTCACCGAACTGC | ||||
| nalD | nalD-F | GCGGCTAAAATCGGTACACT | 789 | 55 | 4 |
| nalD-R | ACGTCCAGGTGGATCTTGG | ||||
| mexR | mexR20 | CCAGTAAGCGGATAC | 1016 | 51 | 4 |
| mexRINT | GGATGATGCCGTTCACCTC | ||||
| mexT | mexT-F | TGCATCACGGGGTGAATAAC | 1398 | 55 | 4 |
| mexT-R | GGTAGCGCCAGGAGAAGTG | ||||
| mexS | mexS-F | ATACAGTCACAACCCATGA | 1153 | 50 | 4 |
| mexS-R | TCAACGATCTGTGAATCT | ||||
| mexZ | mexZ2060 | CCAGCAGGAATAGGGCGACCAGGGC | 1059 | 64 | 4 |
| mexZ1026 | CAGCGTGGAGATCGAAGGCAGCCGG | ||||
| oprD | oprD-F | GGCAGAGATAATTTCAAAACCAA | 1384 | 64 | 26 |
| oprD-R | GTTGCCTGTCGGTCGATTAC | ||||
| oxa50 | oxa50-F | AATCCGGCGCTCATCCATC | 619 | 54 | 32 |
| oxa50-R | GGTCGGCGACTGAGGCGG | ||||
| ges | ges-F | GTTTTGCAATGTGCTCAACG | 371 | 55 | 26 |
| ges-R | TGCCATAGCAATAGGCGTAG | ||||
| imi | imi-F | ATAGCCATCCTTGTTTAGCTC | 818 | 55 | 26 |
| imi-R | TCTGCGATTACTTTATCCTC | ||||
| gim | gim-F | TCGACACACCTTGGTCTGAA | 477 | 55 | 26 |
| gim-R | AACTTCCAACTTTGCCATGC | ||||
| aac(3)-Ia | aac(3)Ia-F | CCCTGACCAAGTCCAATCCATGC | 435 | 55 | 28 |
| aac(3)Ia-R | GGTGGCGGTACTTGGGTCGATA | ||||
| aac(3)-Ic | aac(3)Ic-F | CTCTCAAGACGTTGGTGTAATGC | 143 | 55 | 28 |
| aac(3)Ic-R | CAGCGATTGCGATGAAGCCAGA | ||||
| aac(6″)-Ib | aac(6″)Ib-F | GGTATGCCCAGTCGTACGTTGC | 281 | 55 | 28 |
| aac(6″)Ib-R | TGGACCATMTGGGGTGGTTACG | ||||
| ant(2″)-Ia | ant(2″)Ia-F | ATGAGCGAAATCTGCCGCTCTG | 150 | 55 | 28 |
| ant(2″)Ia-R | GCCCGCCGAGCATTTCAACTAT | ||||
| mcr1 | mcr1-F | AGTCCGTTTGTTCTTGTGGC | 1626 | 58 | 29 |
| mcr1-R | AGAT CCTTGGTCTCGGCTTG | ||||
| mexB | mexB-F | CAACATCCAGGACCCACTCT | 167 | 60 | 7 |
| mexB-R | AGGAAATCTGCACGTTCTGC | ||||
| mexD | mexD-F | CTACCCTGGTGAAACAGC | 250 | 58 | 8 |
| mexD-R | AGCAGGTACATCACCATCA | ||||
| mexF | mexF-F | TGTACGCGAACGACTTCAAC | 163 | 60 | 7 |
| mexF-R | GAGGTGTCGCTGACCTTGAT | ||||
| mexY | mexY-F | TCAGGCCGACCTTGAAGTAG | 159 | 60 | 7 |
| mexY-R | TCTCGGTGTTGATCGTGTTC | ||||
| rpsL | rpsL-F | TACTTCGAACGACCCTGCTT | 163 | 60 | 7 |
| rpsL-R | TTTCCTCGTACATCGGTGGT |