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. 2022 May 24;2022:1889632. doi: 10.1155/2022/1889632

Figure 1.

Figure 1

Generation of Idol knockout hamsters with hypotriglyceridemia. (a) Schematic diagram for the sgRNA targeting site at hamster Idol loci. The sgRNA target sequence is labeled in red. Letters in blue are the protospacer adjacent motif (PAM). (b) Sequencing of the targeted site in the three mutant founders. (Δ: deletion mutation; In: insertion mutation). (c) Genotyping of Idol knockout hamsters with 10 nt deletion. Lane 1: WT hamster (176 bp); lane 2: heterozygous Idol knockout hamster (+/-: 176 bp and 166 bp); lane 3: homozygous Idol knockout hamster (-/-: 166 bp). NS: nonspecific band. (d) Detection of Idol mRNA expression in the liver and small intestine. n = 5-7/group, ∗p < 0.05 and ∗∗p < 0.01 compared with WT; #p < 0.05 compared with Idol+/-. (e) Plasma total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), and triglyceride (TG) levels from 3-month-old male WT and Idol−/− hamsters. n = 10-12/group, ∗∗∗p < 0.001.