TABLE 1.
Quantification and percentage of R. albus, R. flavefaciens, and F. succinogenes 16S rRNA in cecal contents of ponies and donkeys targeted with specific oligonucleotide probes
| Animal | Universal probe (quantification)a | R. albusb(% RNA)c |
F. succinogenes
|
R. flavefaciens
|
||
|---|---|---|---|---|---|---|
| Quantification | % RNA | Quantification | % RNA | |||
| Donkey1 | 35.72 | <0.01 | 0.25 | 0.70 | 0.68 | 1.90 |
| Donkey2 | 29.15 | <0.01 | 0.06 | 0.21 | 2.68 | 9.19 |
| Donkey3 | 25.10 | <0.01 | 0.28 | 1.12 | 1.23 | 4.90 |
| Pony1 | 32.59 | <0.01 | 1.40 | 4.30 | 1.53 | 4.69 |
| Pony2 | 38.87 | <0.01 | 0.27 | 0.69 | 0.74 | 1.90 |
| Pony3 | 17.83 | <0.01 | 0.05 | 0.28 | 0.64 | 3.59 |
a
Quantification is expressed in micrograms of 16S rRNA per 50 mg of lyophilized cecal contents.
b
R. albus was detected at very low levels and was expressed only as a percentage of total 16S rRNA.
c
% RNA is the percentage of total bacterial 16S rRNA.