TABLE 1.
Identification of nisin-producing strains by the nisin bioluminescence assay
| Strain | Luciferase activitya at:
|
Nisin producer | ||
|---|---|---|---|---|
| 1:1 | 1:100 | 1:10,000 | ||
| Lactobacillus sp. | 0.2 | 1.0 | 1.0 | − |
| Pediococcus sp. | 1.0 | 0.9 | 1.1 | − |
| Lactococcus sp. | 0.9 | 1.0 | 1.0 | − |
| Nisin Z producer | 60.5 | 1.7 | 1.1 | + |
| Nisin A producer | <0.1 | 16.2 | 1.2 | + |
| Variant of nisin A | <0.1 | 15.4 | 1.0 | + |
| Unknown test microorganism | <0.1 | 17.6 | 1.1 | + |
a
The values indicate the ratio of the mean activities of the diluted duplicate supernatants compared with that of the uninduced indicator strain LAC182.