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. 2022 May 23;21(6):710–720. doi: 10.1038/s41563-022-01251-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 4 — a, PEGylated LND with and without PEG molecules represented. b, PEGylated liposome without and with cross-sectional view of liposome interior. c, DLS analysis of LND size distributions before and after diffusion through 50 nm pore diameter membranes. d, LND-CDN or CDN-PEG-lipids were incubated with 10% serum in a dialysis cassette with a 5 KDa MWCO membrane, and STING activation bioactivity remaining in the sample (as assessed by activation of RAW-ISG reporter cells) was measured over time (n = 3 biologically independent samples per timepoint, mean±s.e.m. is plottted).