Extended Data Fig. 8. Flow cytometry gating strategy for identifying tumour endothelial cells, tumour cells, and myeloid cells.

a, Flow cytometry gating strategy to identify tumour endothelial cells (CD45⁻CD31⁺ CD146⁺) and non-endothelial tumour cells (all other CD45⁻ cells) is show (see methods for details on tumour digestion and the antibodies used for staining). b, Gating strategy to identify tumour myeloid subsets referred to as CD11b⁺CD11c⁻ cells (CD45⁺ Ly6G⁻ CD11b⁺ CD11c⁻) and CD11c⁺ CD11b⁻cells [CD45⁺ Ly6G⁻DUMP(CD19 CD3e NK1.1)^- CD11c⁺ CD11b⁻] is show (see methods for details on tumour digestion and the antibodies used for staining).