Culturability and viability of A. tumefaciens. Exponentially growing cells were inoculated into AB salts containing various concentrations of cupric sulfate. At various times after inoculation (time zero), aliquots were removed and the numbers of culturable (□) and viable (◊) cells were determined as described in Materials and Methods. Panels: A, microcosm without cupric sulfate; B, microcosm containing 0.05 mM cupric sulfate; C, microcosm containing 0.5 mM cupric sulfate; D, microcosm containing 5 mM cupric sulfate. The error bars represent 1 standard deviation; in most cases, the error bars are too small to be visible.