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. 2022 May 31;13:3013. doi: 10.1038/s41467-022-30621-z

Fig. 5. In vivo administration of ω-3 epoxides improves hypoxic PH and Sugen/hypoxia PH.

Fig. 5

a Representative image of EVG staining (top) and Immunostaining of Vimentin and CD31 (bottom) in lungs of hypoxia-exposed WT mice and Pafah2 KO mice when administered PBS, DHA (0.05 mg/kg/day), or 19,20-EpDPE (0.05 mg/kg/day) i.p. every day. These administrations were started 2 weeks after hypoxic exposure. Scale bar, 50 μm. b–d The evaluation of PH severity in hypoxia-exposed WT mice and Pafah2 KO mice when administered PBS, DHA, or 19,20-EpDPE (WT mice, n = 6,6,7; Pafah2 KO mice, n = 7,6,7). Wall thickness of pulmonary arterioles (b), RVSP (c), weight ratio of RV to LV + septum (d). Experiments were repeated twice and the data were pooled (c, d). e Representative image of EVG staining (top) and Immunostaining of Vimentin and CD31 (bottom) in lungs of Sugen/hypoxia treated WT mice when administered vehicle, DHA (0.05 mg/kg/day), or 19,20-EpDPE (0.05 mg/kg/day) i.p. every day. These administrations were started 3 weeks after hypoxic exposure. Scale bar, 50 μm. f–h The evaluation of PH severity in Sugen/hypoxia treated WT mice when administered PBS, DHA, or 19,20-EpDPE (n = 6,5,6). Wall thickness of pulmonary arterioles (f), RVSP (g), weight ratio of RV to LV + septum (h). Data are mean ± SEM. P values were determined by one-way ANOVA with Dunnett’s post hoc test.