Author Correction: Osthole improves function of periodontitis periodontal ligament stem cells via epigenetic modification in cell sheets engineering

Correction to: Scientific Reports 10.1038/s41598-017-05762-7, published online 12 July 2017

This Article contains errors in Figure 1 and Figure 2.

Figure 1.

Osthole reverses defective osteogenic ability of P-PDLSCs. (A) ALP staining and ARS staining after 7 days (ALP staining) and 21 days (ARS staining) in H-PDLSCs and P-PDLSCs with different concentrations of Osthole (0 Mol/L, 10⁻⁴ Mol/L, 10⁻⁵ Mol/L, 10⁻⁶ Mol/L, 10⁻⁷ Mol/L and 10⁻⁸ Mol/L). (B) Quantification of ARS staining for light absorbance at 562 nm. (C) Gene expression of Runx2, ALP and Osterix in H-PDLSCs, P-PDLSCs and P-PDLSCs with 10⁻⁷ Mol/L Osthole as assayed by qRT-PCR. (D) Protein expression of Runx2, ALP and Osterix in H-PDLSCs, P-PDLSCs and P-PDLSCs with 10⁻⁷ Mol/L Osthole as assayed by Western blot. *P < 0.05, **P < 0.01, ns: P ≧ 0.05, n = 3.

Figure 2.

Osthole reverses defective osteogenesis of P-PDLSCs through histone acetylation. (A) ALP staining and ARS staining of H-PDLSCs, P-PDLSCs and P-PDLSCs with 10⁻⁷ Mol/L Ostholes in P4 (with stimulation), P6 (without stimulation) and P10 (without stimulation). (B) Quantification of ARS staining for light absorbance at 562 nm. (C) qRT-PCR showed gene expression of fifteen histone acetylases in H-PDLSCs and P-PDLSCs. (D) Protein expression of MOZ, MORF, H3K9ac and H3K14ac in H-PDLSCs and P-PDLSCs as assayed by western blot. (E) qRT-PCR showed gene expression of fifteen histone acetylases in P-PDLSCs and P-PDLSCs with 10⁻⁷ Mol/L Osthole measured by qRT-PCR. (F) Protein expression of MOZ, MORF, H3K9ac and H3K14ac in P-PDLSCs with 10⁻⁷ Mol/L Osthole treatment on day 0, 3, 5, 7, 10 as assayed by western blot. (G) Level of acetylation of H3K9 and H3K14 in P-PDLSCs with 10⁻⁷ Mol/L Osthole treatment on day 0, 3, 5, 7, 10 as assayed by EpiQuik Global Acetyl Histone Quantification Kit. *P < 0.05, **P < 0.01, ***P < 0.001, no mark: P ≧ 0.05, n = 3.

In Figure 1A, the ARS staining images in the lower row for H-PDLSC+DMSO and P-PDLSC+Osthole, 10⁻⁸ Mol/L are incorrect. Furthermore, in Figure 2A, the APS straining image for H-PDLSC+DMSO in P6 is incorrect. The correct Figure 1 and Figure 2 and accompanying legends appear below.