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. 2022 May 31;13(5):511. doi: 10.1038/s41419-022-04910-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A Scheme. mice were treated with GAP26 by i.p. injection after UUO surgery. B RT-qPCR. Levels of mRNA encoding α-SMA, Fibronectin by RT-qPCR. C PAS and immunofluorescence staining. PAS staining was performed to evaluate renal tubular injury by calculating tubules about GAP26. α-SMA is a marker of myofibroblast. Corresponding graphs indicate the expression of α-SMA for evaluation of myofibroblast infiltration. Data are presented as the mean ± SEM, n = 4–7/group, *P < 0.05, **P < 0.01, ***P < 0.001; Scale bar: 100 μm.