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. 2022 May 31;13(5):511. doi: 10.1038/s41419-022-04910-w

Fig. 3. Knockout the Cx43 gene of TECs prevents UUO-induced renal injury and RIF.

Fig. 3

A, B Experiment schematic and Levels of mRNA encoding Cx43 by RT-qPCR. C PAS staining was performed to evaluate renal tubular injury by calculating damaged tubules with dilation swelling or epithelial cell brush border loss. The red area in SR staining indicates extracellular fibrotic collagen deposition in renal interstitium. D Immunofluorescence. Representative photomicrographs for immunofluorescence labeled Cx43 (green), α-SMA (red), and FN (green). Corresponding graphs indicate the expression of Cx43 (green), α-SMA (red) and FN (green). E RT-qPCR. Levels of mRNA encoding α-SMA, Fibronectin, Collagen Iα1 by RT-qPCR. Data are presented as the mean ± SEM, n = 3–6/group, *P < 0.05, **P < 0.01, ***P < 0.001; Scale bar: 100 μm.