Figure 4. DDX5-dependent tuft cell gene programs in the small intestine.

A. Representative electron microscopy images of small intestinal tuft cells from WT^IEC and DDX5^ΔIEC mice. Scale bar: 5μm.

B. Loupe browser screenshots displaying quantified Dclk1^hi spots on the small intestinal sections from WT^IEC and DDX5^ΔIEC mice described in Figure 2A. Scale bar: 2mm.

C. Gene ontology analysis revealed the top 7 pathways downregulated (black) or upregulated (red) in DDX5^ΔIEC Dclk1^hi cells from B.

D. Violin plots displaying expressions of DDX5-dependent genes—with or without DDX5-associated transcripts—involved in the antimicrobial response (Defa3 and Defa17), viral response (Duox2 and Hspb1), transport (Slc6a19), proteolysis (Anpep and Cnbp2), or lipid metabolism (Apoa1) in WT^IEC and DDX5^ΔIEC Dclk1^hi cells from C.

E. Log fold changes (DDX5^ΔIEC/ WT^IEC) in the expression of tuft cell DDX5-dependent genes participating in pathways identified in C. Small intestinal IEC DDX5-associated transcripts identified in eCLIP analysis are indicated with a blue star (GSE124023).