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. 2022 May 31;13:3030. doi: 10.1038/s41467-022-30521-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Representative ISFs f(q, Δt) of liquid-like, high-diffusive (green curve), and low-diffusive (blue curve) droplets, and dynamically arrested condensates (gray squares) observed in samples containing various Dhh1/polyU ratios. b–d Increase of the fraction of dynamically arrested droplets at low Dhh1/polyU ratios, as measured by DDM (b, c), which is consistent with the increase in the fusion time T_fus of the condensates (in d). In panel c, numbers indicate the fraction of arrested droplets in %. A high fraction of dynamically arrested droplets is highlighted in red, a low fraction is highlighted in green. The color bar in panel d describes the absolute polyU concentration used in panels b, d. In b, c, the fraction of arrested droplets was calculated by analyzing at least 15 different condensates in three independent samples. Source data for panels b–d are provided in the Source Data file.