Table 1.
MICs for E. coli BW25113 strains harboring a pBAD18 plasmid which contains a tet(X2), tet(X3), tet(X4), mutated tet(X2) gene, mutated tet(X3) gene or mutated tet(X4) gene.
| E. coli Strains | MIC (mg/L) | ||
|---|---|---|---|
| TGC | MIN | TETa | |
| E. coli 25922 | 0.25 | 0.25 | 1 |
| Vector control | 0.5 | 1 | 2 |
| tet(X2) | 1 | 4 | 16 |
| tet(X3) | 16 | 16 | 128 |
| tet(X4) | 16 | 16 | 128 |
| H196Lb | 1 | 4 | 32 |
| Q197H | 1 | 2 | 32 |
| S217A | 2 | 4 | 32 |
| S217G | 1 | 2 | 16 |
| H279R | 1 | 4 | 16 |
| T280V | 2 | 4 | 16 |
| T280L | 1 | 4 | 16 |
| T280S | 2 | 4 | 16 |
| L282S | 4 | 8 | 32 |
| E295G | 1 | 4 | 32 |
| E295N | 2 | 4 | 32 |
| E295D | 1 | 4 | 16 |
| K296N | 1 | 2 | 16 |
| P297D | 1 | 2 | 8 |
| P297S | 1 | 4 | 16 |
| I359M | 1 | 4 | 16 |
| I359V | 1 | 4 | 32 |
| V329L | 2 | 8 | 32 |
| V329M | 2 | 8 | 32 |
| A339T | 2 | 4 | 32 |
| D340N | 1 | 4 | 32 |
| V350I | 1 | 4 | 32 |
| K351E | 2 | 4 | 32 |
| A339T/D340N | 4 | 8 | 64 |
| V350I/K351E | 4 | 8 | 64 |
| V329L/A339T/D340N | 8 | 8 | 64 |
| V329L/V350I/K351E | 4 | 8 | 64 |
| V329M/A339T/D340N | 4 | 8 | 128 |
| V329M/V350I/K351E | 8 | 16 | 64 |
| A339T/D340N/V350I/K351E | 8 | 8 | 64 |
| V329L/A339T/D340N/V350I/K351E | 16 | 16 | 128 |
| V329M/A339T/D340N/V350I/K351E | 16 | 16 | 64 |
| tet(X3)-L329V/T339A/N340D/I350V/E351Kc | 2 | 8 | 64 |
| tet(X4)-M329V/T339A/N340D/I350V/E351Kd | 2 | 8 | 64 |
aTET tetracycline, MIN minocycline, TGC tigecycline.
bAll mutants are derived from pBAD18-Tet(X2) by site-directed mutagenesis.
ctet(X3)-L329V/T339A/N340D/I350V/E351K is reverse penta mutant derived from pBAD18-tet(X3).
dtet(X4)-M329V/T339A/N340D/I350V/E351K is reverse penta mutant derived from pBAD18-tet(X4).