Table 2.
Kinetic parameters (± SD) of the TetX proteins on tigecycline.
| Protein | kcat (S−1) | Km (µM) | kcat/Km (M−1 S−1) |
|---|---|---|---|
| Tet(X2) | 1.04 ± 0.01 | 4.45 ± 0.13 | 2.33 × 105 |
| Tet(X3) | 2.16 ± 0.05 | 2.97 ± 0.2 | 8.09 × 105 |
| Tet(X4) | 2.00 ± 0.02 | 1.73 ± 0.09 | 1.16 × 106 |
| S217A | 2.13 ± 0.07 | 4.58 ± 0.41 | 4.65 × 105 |
| T280V | 0.76 ± 0.01 | 3.02 ± 0.16 | 2.51 × 105 |
| T280S | 1.15 ± 0.03 | 3.09 ± 0.24 | 3.72 × 105 |
| L282S | 1.36 ± 0.05 | 3.96 ± 0.38 | 3.43 × 105 |
| E295N | 1.39 ± 0.07 | 4.92 ± 0.64 | 2.83 × 105 |
| V329L | 0.97 ± 0.03 | 3.56 ± 0.35 | 2.72 × 105 |
| V329M | 3.80 ± 0.11 | 5.18 ± 0.34 | 7.34 × 105 |
| A339T | 1.95 ± 0.11 | 3.69 ± 0.50 | 5.28 × 105 |
| K351E | 6.82 ± 0.40 | 3.48 ± 0.47 | 1.96 × 106 |
| A339T/D340N | 0.95 ± 0.05 | 3.81 ± 0.49 | 2.49 × 105 |
| V350K/I351E | 1.64 ± 0.01 | 3.51 ± 0.54 | 4.67 × 105 |
| V329L/A339T/D340N | 1.29 ± 0.03 | 1.05 ± 0.08 | 1.23 × 106 |
| V329L/V350I/K351E | 1.13 ± 0.03 | 2.22 ± 0.17 | 5.09 × 105 |
| V329M/A339T/D340N | 3.94 ± 0.14 | 4.25 ± 0.39 | 9.27 × 105 |
| V329M/V350I/K351E | 1.99 ± 0.06 | 2.81 ± 0.24 | 7.08 × 105 |
| A339T/D340N/V350I/K351E | 1.69 ± 0.06 | 2.89 ± 0.30 | 5.85 × 105 |
| V329L/A339T/D340N/V350I/K351E | 1.38 ± 0.03 | 2.03 ± 0.13 | 6.79 × 105 |
| V329M/A339T/D340N/V350I/K351E | 1.95 ± 0.13 | 3.53 ± 0.52 | 5.52 × 105 |
| Tet(X3)-L329V/T339A/N340D/I350V/E351K | 0.82 ± 0.02 | 2.65 ± 0.29 | 3.09 × 105 |
| Tet(X4)-L329V/T339A/N340D/I350V/E351K | 2.89 ± 0.13 | 3.95 ± 0.61 | 7.31 × 105 |