Fig. 5. Reduction of hepatic IKK-β, cleavage and Ser166-dephosphorylation of RIPK1 during apoptosis induced by d-GalN/LPS does not occur in Bcl-3^Hep mice.

A Immunoblotting was performed with a specific antibody against the C terminus of IKK-β in whole liver tissue lysates from Bcl-3^Hep and WT mice at 4 h after d-GalN/LPS challenge and corresponding controls. B To test the involvement of RIPK1 in d-GalN/LPS-induced liver injury, RIPK1 protein levels were analyzed in cytosolic (cyt.) and mitochondrial (mit.) protein fractions from two representative Bcl-3^Hep and WT mice treated with d-GalN/LPS. Phospho-RIPK1 (Ser166) levels were determined in whole liver homogenates from Bcl-3^Hep and WT mice at 4 h post D-GalN/LPS or PBS injection. In A and B representative western blots with densitometric analysis are shown. Tubulin and GAPDH served as protein loading controls. **p < 0.01 for WT vs. Bcl-3^Hep according to an unpaired, two-tailed Student’s t-test (A and B).