Fig. 6. Agonistic anti-FAS (CD95/APO-1) antibody Jo2-induced acute liver injury is attenuated in Bcl-3^Hep mice.

Liver injury was assessed by measurement of A serum ALT and AST levels and B caspase 3 activity on liver histology at 4 h post Jo2. C Hepatic JNK activity and D IKK-β expression were analyzed by immunoblotting in whole liver tissue lysates. Data in A represent means ± SEM from two experiments with a total of n = 8 WT + Jo2, n = 8 Bcl-3^Hep + Jo2, n = 7 WT + PBS, and n = 7 Bcl-3^Hep + PBS. In B representative histological photomicrographs (scale bar: 2000 µM) are depicted. In C and D representative western blots with densitometric analysis are shown. **p < 0.01, ***p < 0.001 for WT vs. Bcl-3^Hep and ^$$p < 0.01, ^$$$p < 0.001 for PBS vs. Jo2 using Mann–Whitney U test (A: ALT) or unpaired, two-tailed Student’s t-test (A: AST and B–D). In C there was no statistical difference between the two groups in respect of JNK activation.