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. 2022 May 31;13:3023. doi: 10.1038/s41467-022-30598-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — The crystal structure of the Streptococcus pyogenes Cas9 poised to cleave DNA (PDB accession code 5F9R)²¹ with the HNH and RuvC nuclease domains highlighted in green and blue, respectively. The inset shows the three regions of the HNH domain targeted for computational mutagenesis and the conceptual approach is shown schematically.