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Schematic representation of the construction of the pIMP1eglATNF and the pKNT19eglATNF shuttle vector and of the mutations introduced in the original eglA sequence and the mTNF-α cDNA. PeglA, promoter region of eglA; SSeglA, signal sequence of eglA; SD, Shine-Dalgarno sequence; SPase, signal peptidase cleavage site (indicated by ↑).
