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. 2022 Apr 25;41(11):e109985. doi: 10.15252/embj.2021109985

Figure 1. HF induces an atypical blunted ISR.

Figure 1

  1. Representative immunoblots of the indicated proteins in lysates from HeLa cells treated with indicated concentrations of halofuginone (HF) for 5 h.
  2. Quantification of ATF4 and P‐eIF2α from experiments as in (A). Data are mean ± SD (n = 3 biological replicates). *P ≤ 0.0414, **P ≤ 0.0019, ****P < 0.0001, as determined by one‐way ANOVA with Dunnett’s multiple comparison test.
  3. Similar to (A), but with HeLa cells treated with indicated concentrations of tunicamycin for 5 h.
  4. Similar to (A), but using lysates from HeLa cells treated with 62.5 nM and 312.5 nM HF for indicated times.
  5. Relative abundance of the indicated mRNAs detected by qPCR in lysates from HeLa cells treated with indicated concentrations of HF for 5 h. Data are mean ± SD (n = 3 biological replicates). *P ≤ 0.0388, **P ≤ 0.0046, ***P ≤ 0.0008, as determined by one‐way ANOVA with Dunnett’s multiple comparison test.

Data information: Representative results of at least three independent experiments are shown in each panel.