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. 2022 Apr 25;41(11):e109985. doi: 10.15252/embj.2021109985

Figure 2. GCN2 mediates eIF2α phosphorylation by HF.

Figure 2

  1. Representative immunoblots of indicated proteins in lysates from mouse embryonic fibroblasts (MEFs) of indicated genotype after treatment with 12.5 nM or 200 nM HF for 5 h.
  2. Similar to (A), but using HeLa cells untreated or treated with GCN2 siRNA 48 h before treatment with indicated concentrations of HF for 5 h.
  3. Similar to (A), but with high HF concentrations.

Data information: Representative results of at least three independent experiments are shown in each panel.