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. 2022 Apr 25;41(11):e109985. doi: 10.15252/embj.2021109985

Figure EV2. The Nedd8‐activating enzyme inhibitor MLN4924 stabilizes ATF4 and R15B.

Figure EV2

  1. Representative immunoblots of the indicated proteins in lysates from HeLa cells treated with cycloheximide (20 μg/ml) with or without the inhibitor of the NEDD8‐activating enzyme MLN4924 (1 μM) for indicated times.
  2. Quantification of R15B and ATF4 in cells treated with MLN4924 (1 μM) for 120 min. Data are mean ± SEM (n = 3 biological replicates). *P = 0.0369, as determined by unpaired t‐test.
  3. Quantification of experiments such as the ones shown in (A). t1/2 values were calculated with results from 3 independent experiments. Data are mean ± SEM (n = 3 biological replicates).