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CH3Cl utilization and formate formation by cell extracts of strain CC495. Cell extracts were incubated at 25°C with 50 mM phosphate buffer (pH 7.8) containing 1 mM NADH, 0.5 mM DTT, and 17.2 mM CH3Cl. Formate concentrations (■) were determined by GC-MS, and CH3Cl concentrations (●) were determined by gas chromatography.
