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SDS-PAGE of protein fractions from stages in the purification of methyltransferase from strain CC495. The gel was stained with Coomassie blue. Lane 1, 0.55 μg of protein from the gel filtration stage; lane 2, 0.69 μg of protein from the anion-exchange stage; lane 3, 0.46 μg of protein from crude cell extracts; lane 4, protein standards with molecular masses (in kilodaltons) as indicated.
