Figure 2.

CCL27a-knockout has little effect on the establishment of CCR10⁺ lymphocytes in the skin

(A) Representative FC analysis of gated skin CD45⁺ immune cells for T cell subsets and ILCs in adult CCL27a^−/− and CCL27a^+/+ mice.

(B) Percentages of T cell and ILC populations from CCL27a^−/− versus CCL27a^+/+ or CCL27a^+/− adult mouse skin. N = 7 mice each for CCL27a^−/− and control groups. Unpaired one-tailed Student’s t-test.

(C) Representative FC analysis of skin T cell subsets and ILCs from adult CCL27^−/−CCR10^+/EGFP and CCL27a^+/+CCR10^+/EGFP mice gated for their CCR10(EGFP)⁺ subpopulations. CD8⁺ and CD4⁺ T cells are gated on CD8⁺ and CD4⁺ CD45⁺Thy1.2⁺CD3^int+ populations respectively. ILCs are CD45⁺Th1.2⁺CD3⁻. γδT cells are CD45⁺Thy1.2⁺CD3^int+TCRδ⁺ (excluding CD3^high dendritic epidermal γδT cells).

(D) Comparative percentages of CCR10⁺ subsets of indicated skin lymphocyte populations from CCL27a^−/−CCR10^+/EGFP versus CCL27a^+/+CCR10^+/EGFP or CCL27a^+/−CCR10^+/EGFP adult mice. N = 6 mice each for CCL27a^−/− and control group. Unpaired one-tailed Student’s t-test.