Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 May 18;25(6):104426. doi: 10.1016/j.isci.2022.104426

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Impaired establishment and dysregulated localization of CCR10⁺ lymphocytes in the skin of CCL27^−/− mice

(A and B) Numbers of αβT cells (A) and percentages of CCR10(EGFP)⁺ Treg, αβT, γδT, cells and ILCs (B) in the skin of two-week-old CCL27^+/+CCR10^+/EGFP and CCL27^−/−CCR10^+/EGFP mice. N = 5 mice for CCL27^+/+ and four for CCL27^−/− samples. The cell number is calculated from FC analysis of cell preparations of the whole trunk skin.

(C) Percentages and cell counts of αβT cells of skin CD45⁺ cells from adult CCL27^+/+CCR10^+/EGFP and CCL27^−/−CCR10^+/EGFP mice. N = 11 mice for CCL27^+/+CCR10^+/EGFP and 10 for CCL27^−/−CCR10^+/EGFP samples.

(D) Percentages of CCR10(EGFP)⁺ subsets of different skin lymphocyte populations in adult CCL27^+/+CCR10^+/EGFP and CCL27^−/−CCR10^+/EGFP mice. N = 5 mice of each genotype for the CD8⁺, CD4⁺ and γδ T cell data and three each for the ILC data.

(E) Immunofluorescent images of skin sections of 6-week-old CCL27^+/+CCR10^+/EGFP and CCL27^−/−CCR10^+/EGFP mice for CCR10(EGFP)⁺ lymphocytes and T cells. Arrowheads identify CCR10(EGFP)⁺ cells. The green long rod-like structures are autofluorescent hairs. Super-bright CD3⁺ cells are DETCs.

(F) Relative percentages of CCR10(EGFP)⁺ lymphocytes and T cells close to or below hair follicle structures in CCL27^+/+CCR10^+/EGFP versus CCL27^−/−CCR10^+/EGFP mice, based on calculation of total 1078 and 943 CCR10⁺ cells, respectively. Representative of four CCL27^+/+CCR10^+/EGFP and CCL27^−/−CCR10^+/EGFP gender-matched littermate adult mice with 20 images analyzed per mouse. One tailed paired t-test. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001.