REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-mouse CD45	Biolegend	30-F11
anti-mouse TCRδ	Biolegend	Clone GL3
anti-mouse CD3,	Biolegend	17A2
anti-mouse CD4	Biolegend	GK1.5
anti-mouse CD8	Biolegend	53-6.7
anti-mouse Thy1.2	Biolegend	30-H12
anti-mouse IL-17A	Biolegend	TC11-18H10.1
anti-mouse TCRβ	Biolegend	H57-597
anti-mouse CD45.2	Biolegend	104
anti-Sca1	Biolegend	E13-161.7
anti-EpCAM	Biolegend	G8.8
anti-I-A/I-E	Biolegend	M5/114.15.2
anti-CD45	Biolegend	30-F11
and anti-mouse CD45.1	Biolegend	A20
Anti-mouse/human IL-22	eBioscience	IL22JOP
anti-mouse FoxP3	eBioscience	FJK-16s
Anti-mouse CD34	BD Biosciences	RAM34
goat anti-mouse CCL27 antibodies	R&D systems	AF725
Control goat IgG antibody	R&D systems	AB-108-C
Alexa Fluor 647 chicken anti-goat antibody	Invitrogen	A-21469
Chemicals, peptides, and recombinant proteins
Imiquimod	Perrigo	NDC 45802-368-00
DAPI	Biolegend	422801
Prolong Diamond Antifade	Invitrogen	P36961
Critical commercial assays
BaseScope Reagent Kit-Red	Advanced Cell Diagnostics, INC	Cat #: CAT NO: 323600
Experimental models: Organisms/strains
CCL27a-KO mice	UC-Davis KOMP Repository	C57BL/6N-CCL27a tm1(KOMP)Vlcg
Total CCL27-KO mice	Generated in lab with help of the Gene Modification Facility of the Albert Einstein College of Medicine.	None
CCR10-KO/EGFP-KI mice	Generated in lab	None
Oligonucleotides
CCL27 RNA probe: 5′TTGCTTCTGAGCCCGGC TCCTGAAGCAGCCTTGCCTCTGCCCTCCAGC	Advanced Cell Diagnostics, INC	None
Primers for qRT-PCR of total CCL27 mRNA: (F)5′GA TGGGGACTGTCACCTCCAG, (R)5′CCTTGGAGCC TTTTCCCTTGGCGT	IDT	None
Primers for qRT-PCR of CCL27a mRNA: (F)5′CACA TGGAACTGCAGGAGGCC, (R)5′TGTAGTACCAGA TTTAAACTGGGTACAGTC	IDT	None
Primers for qRT-PCR of CCL27b mRNA: (F)5′CACA TGGAACTGCAGGAGGCT, (R)5′TGTAGTACCAGA TTTAAACTGGGTACAGTT	IDT	None
Primers for qRT-PCR of CCL28 mRNA: (F)5′CAGCC CGCACAATCGTACT, (R)5′ACGTTTTCTCTGCCAT TCTTCTTT	IDT	None
Primers for qRT-PCR of β-actin mRNA: (F)5′CT GTCGAGTCGCGTCCA, (R)5′CACGATGGAG GGGAATACAGC	IDT	None
Primers for qRT-PCR of GAPDH mRNA: (F)5′A GGTCGGTGTGAACGGATTTG, (R)5′TGTAGA CCATGTAGTTGAGGTCA	IDT	None
Primers for PCR genotyping of total CCL27-KO mice: (F)5′GCCAAGAGTTAGAGCTCAGCTTC, (R)5′GGTGACAGTCCCCATCGG	IDT	None
Primers for PCR genotyping of CCL27a-WT allele: (F)5′CTTTAGCGCCGCAGCCGCCT, (R)5′CTATAGAAAGGACCTTGGACCCTCA	IDT	None
Primers for PCR genotyping CCL27a-KO allele: (F)5′ACTTGCTTTAAAAAACCTC CCACA, (R)5′CTGTGGATGGAGGTCTCAGCTGA	IDT	None