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. 2022 Mar 16;189(2):687–702. doi: 10.1093/plphys/kiac106

Figure 2.

Figure 2

Cuscuta campestris grows without a plant host under the controlled conditions of the AHS. A–F, Cuscuta campestris 3 cm shoot tips were used as inoculum. A, 3 cm shoot tips attached to an AH at 0 dpi. B, Parasite coiled with holdfasts after 2.5 dpi. C, Parasite at 15 dpi, growth is evident as flower development (bottom arrow) in plant No. 1 and production of a new shoot (top arrow) in plant No. 2. D, New shoots elongated, 36 dpi. E, Parasite flowered and produced seeds (inset; scale is in millimeters). F, Cuscuta campestris attached to an AH without any media or water at 36 dpi. Parasite did not develop swollen holdfast or subsequent growth. G, Seedlings after scarification and germination of seeds obtained from C. campestris grown in the AHS. H, Seedling from seed obtained through AHS colonizing Arabidopsis 10 dpi. I, Seedling growing 36 dpi after being used as inoculum in a second AHS.