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. 2022 Mar 18;189(2):1128–1138. doi: 10.1093/plphys/kiac131

Figure 1.

Figure 1

Identification of an acquired osmotolerance-defective mutant (aod13) of A. thaliana. A, Flow chart of the acquired osmotolerance assay (top). Salt-acclimated 2-week-old seedlings of accession Bu-5 were mesh-transferred to MS agar plates containing 750 mM sorbitol for 21 d. The seedlings showing osmo-hypersensitivity (red circle) were screened as aod mutants. Impaired acquired osmotolerance of the aod13 mutant (bottom). WT Bu-5 is shown as an example of acquired osmotolerance and accession Col-0 is shown as an example of impaired acquired osmotolerance. Bottom panel: Two-week-old Bu-5 WT, aod13, and Col-0 WT seedlings under normal growth conditions. B, Chlorophyll contents of the seedlings shown in A. FW, fresh weight. C, Osmo-shock tolerance assay comparing aod13 mutant and Bu-5 WT: 2-week-old seedlings were mesh-transferred to MS agar plates containing 600 mM sorbitol for 21 d. D, Chlorophyll contents of seedlings shown in (C). E, Examples of 4-week-old plants grown on soil under normal growth conditions. F, Fresh weight of plants grown as described in (E). Differences between WT (black bar) and aod13 (white bar) were analyzed by Student’s t test (mean ± se, n = 8, ***P < 0.001).