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. 2022 Mar 14;189(2):703–714. doi: 10.1093/plphys/kiac113

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© The Author(s) 2022. Published by Oxford University Press on behalf of American Society of Plant Biologists.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Validation of sgRNA targeting of pAPX2 in a transient transactivation assay in N. benthamiana. LUC-based assay to validate targeting efficiency of sgRNA cassettes to APX2₆₀₀ _bp promoter fragment. Luciferin-infiltrated N. benthamiana leaf 3 d after co-infiltration with pAPX2₆₀₀ _bp::LUC and p2x35S::3xFlag-dCas9-VP64 in combination with sgRNA cassette a (a), cassette b (b), or scrambled cassette (c), or without any cassette (–). High LUC activity in presence of cassette a indicates binding of 3xFlag-dCas9–VP64 to pAPX2_600bp and transactivation of LUC expression by transcriptional activator VP64. The image is representative of three independent experiments.