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. 2022 Mar 14;189(2):703–714. doi: 10.1093/plphys/kiac113

Figure 3.

Figure 3

Effect of dCas9–(d)JMJ on type II transcriptional memory of pAPX2::LUC. A, Treatment scheme for LUC-based type II transcriptional memory assay. Four-day-old seedlings were either exposed to priming HS (P) on Day 4 and imaged on Day 4 (P d4) or on Day 6 (P d6), exposed to a triggering HS (T) on Day 6 and imaged on Day 6 (T), exposed to priming HS on Day 4 and a triggering HS on Day 6 (P + T) and imaged on Day 6 (P + T), or not exposed to any HS and imaged on Day 4 (N d4) or on Day 6 (N d6). B, LUC-based type II transcriptional memory assay shows reduced transcriptional activity of the pAPX2600bp::LUC reporter in dCas9-JMJ lines (top panel, JMJ #1 and JMJ #2) compared to the parent, as well as different behavior of two dCas9–dJMJ lines (middle and bottom, dJMJ #1 and dJMJ #2). For images within one panel the indicated genotypes were grown and treated side-by-side on the same set of plates. Images are representative of three independent experiments.