Skip to main content
. 2022 Mar 14;189(2):703–714. doi: 10.1093/plphys/kiac113

Figure 4.

Figure 4

Type II transcriptional memory of endogenous APX2 and pAPX2::LUC after recurrent HS is reduced in dCas9–JMJ lines. A, Treatment scheme for RT-qPCR-based type II transcriptional memory assay. Four-day-old seedlings were either exposed to priming HS (P) on Day 4, exposed to a triggering HS (T) on Day 6, exposed to a priming and a triggering HS (P + T), or not exposed to any HS (N). All samples were taken on d6 corresponding to the end of the HS treatment. B, Transcript levels of endogenous APX2 (At3g09640), LUC (pAPX2600 bp::LUC), and HSP101 (At1g74310) in parent, two dCas9–JMJ lines (JMJ #1 and JMJ #2), and two dCas9-dJMJ lines (dJMJ #1 and dJMJ #2) as measured by RT-qPCR. Expression values are relative to At4g26410. Data are mean ± sem of three independent experiments. Transcript levels were statistically evaluated for all genotypes within each time point by Tukey’s HSD (P < 0.05). Genotypes are assigned one or more letters based on their statistical group. Genotypes sharing one letter are not significantly different.