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. 2022 Apr 8;12(6):jkac081. doi: 10.1093/g3journal/jkac081

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© The Author(s) 2022. Published by Oxford University Press on behalf of Genetics Society of America.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 4. — Frequency of drive carriers in cage populations. Flies carrying 1 copy of the drive allele and two copies of Cas9 were introduced at initial frequencies of 8.8% (cage 1) and 41.3% (cage 2) into a population that was wild-type at the drive site and homozygous for the Cas9 allele. The cage populations were followed for several nonoverlapping generations, each lasting 12 days, including 1 day of egg laying. All individuals from each generation were phenotyped for DsRed, with positive drive carriers having either 1 or 2 drive alleles (all drive carriers in the initial generation were drive/wild-type heterozygotes).