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Analysis of transcriptome data. mRNA levels from pollen tubes grown under different temperature regimes were investigated by RNA sequencing. A, PCA of RT 3 h, RT 6 h and HS 3 + 3 h. B and C, Volcano plots of RT 3 h versus RT 6 h and RT 6 h versus HS 3 + 3 h, respectively. Red lines indicate threshold for differential expression (abs. log2 FC > 1, outside of vertical lines and FDR < 0.005, above horizontal line). n = 3.
