Figure 4.

Monoacylglycerphospholipid species with saturated acyl chains increase under HS. The depicted lipid subclasses from pollen tubes grown under different temperature regimes were investigated. A, Relative abundances of the lipid subclasses were measured by UPLC-nanoESI-MS/MS and normalized to their respective values after 3 h RT. B–F, Molecular species within the individual subclasses are given as mol % of all detected lipid species per subclass. Only major lipid species (>1% of total) are depicted. G, Relative saturation of the respective lipid classes under the same temperature regimes. The abundance of the molecular lipid species containing zero (saturated), one (monounsaturated), or more than one (polyunsaturated) double bonds in their acyl residues were summed per lipid subclass and converted into relative proportions. n = 5. Error bars represent standard deviation. For statistical analysis, ANOVA was performed (significance level α = 0.05), followed by post hoc Tukey’s analysis. Results are presented as compact letter display of all pair-wise comparisons.