Fig. 2.

Crosses to generate potential drive-resistant cd mutant alleles. Crosses were made with homo- or hemizygous AgNosCd-1 (CFP⁺) males and females to wild-type (outcrosses) or sibling (intercrosses) mosquitoes to detect and monitor the formation and inheritance patterns of mutant cd alleles. Graphic representations of parental and progeny phenotypes (half-circle coloration) and alternative genotypes (marked chromosome symbols) are presented along with phenotypic frequencies. The left half of each circle denotes the eye color phenotype of wild-type (black), cd mutant (red) or mosaic, tear (white slash) in a wild type- or cd-mutant background (black or red, respectively). The right half of the circle denotes negative or positive for CFP fluorescence (black or blue, respectively). The numbers of each phenotype seen and total number of mosquitoes scored are shown in parentheses. Top panels: crosses used to generate mutant cd alleles, a) hemizygous and b) homozygous female CFP⁺ individuals were used to increase the likelihood of recovery of NHEJ-induced mutant cd alleles resulting from maternal effects as seen in Carballar-Lejarazú et al. (2020), with 50 AgNosCd-1 (hemizygous or homozygous) females crossed with 50 wild-type (WT) males in triplicate. Individual experimental details are presented in Supplementary Tables 2 and 3. Bottom panels c and d: crosses with selected progeny obtained in b (dashed box outline labeled “1”) with potential mutant cd alleles to assess the inheritance patterns of mutant cd alleles in the presence or absence of a chromosome carrying the drive system. c) A total of 15 red-eye/CFP⁺ hemizygous males were removed from the hemizygote intercross cage (Cross D) after their mating period, allocated to a new cage and outcrossed to 75 WT females. d) A total of 47 female and male red-eye/CFP⁺ homozygotes generated in b were intercrossed. e) Phenotypes and possible genotypes of progeny from an intercross of ∼60 mixed-sex red-eye/CFP⁻ progeny from d (dashed box outline labeled “2”).