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. 2022 Feb 3;189(2):790–804. doi: 10.1093/plphys/kiac045

Figure 1.

Figure 1

Isolation and analysis of RCII complexes. Room temperature absorption spectra (A), 77 K Chl fluorescence spectra (B) and 2D gel analysis of RCII preparation (C) isolated from cells of the CP47-less strain expressing His-tagged D2. The preparation was isolated using Ni affinity chromatography and analyzed using CN-PAGE in the first dimension. The native gel was photographed (1D color) and scanned by LAS 4000 for fluorescence (1D fluor). After SDS–PAGE in the second dimension, the gels were stained by SYPRO Orange (2D SYPRO stain). RCII* is the D1/D2 complex containing Ycf39/Hlips, RCIIa and RCIIa(2) are monomeric and dimeric forms of the D1/D2 complex lacking the Ycf39/Hlips complex, respectively, and RCIIa/PSI designates the PSI monomer bound to RCIIa. Designated proteins were identified by MS (see Supplemental Table S1); nonspecifically interacting proteins are designated with an asterisk. PsbN and Slr0575 were identified using specific antibodies. About 0.5 µg of Chl was loaded onto the gel.