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. 2022 Feb 3;189(2):790–804. doi: 10.1093/plphys/kiac045

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© The Author(s) 2022. Published by Oxford University Press on behalf of American Society of Plant Biologists.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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HPLC analysis of pigments and heme in the RCII complexes. RCII* and RCIIa complexes (A) were purified by CN-PAGE from the His-D2 preparation and FLAG-D1 and FLAG-D2 preparations (B) were isolated using FLAG affinity chromatography from strains lacking PSII Chl-binding subunits and expressing either FLAG-D1 or FLAG-D2. The analysis was performed as described in “Materials and methods” and the chromatograms were normalized to the highest peak in the preparation; in RCII*, RCIIa, and FLAG-D1 to Chl; in FLAG-D2 to heme.