Table 1.
The stoichiometry of pigment cofactors present in the crude His-D2 preparation and in the RCII*, RCIIa, and RCIIa/PSI complexes
| Preparation | Stoichiometry of Pigment Cofactors |
|||
|---|---|---|---|---|
| Chl-a | β-car | Pheo | Heme-b | |
| Crude His-D2 | 9.7 ± 2.2 | 2.4 ± 0.4 | 1.2 ± 0.3 | 1.0 |
| RCII* | 9.8 ± 1.1 | 2.6 ± 0.6 | 1.8 ± 0.1 | 1.0 |
| RCIIa | 6.7 ± 0.25 | 1.4 ± 0.3 | 1.8 ± 0.05 | 1.0 |
| RCIIa/ PSI | 65.2 ± 0.7 | 7.5 ± 1.2 | 1.9 ± 0.3 | 1.0 |
The complexes were purified from the His-D2/ΔCP47 strain using a combination of Ni affinity chromatography and native gel electrophoresis. The ratio of all pigments is normalized to one heme. Data shown as mean of three independent replicates; see methods for details of pigment quantification.