Table 2.
List of proteins identified by MS specifically in the isolated FLAG-D1/ΔPSII and FLAG-D2/ΔPSII preparation after subtraction of proteins identified in the ΔPSII control
| Preparation | Protein UniProt KB No. | Mass Spectrometric Analysis |
|||
|---|---|---|---|---|---|
| Size (Da) Length (AA) | Coverage (%) | Detected no. of peptides | Intensity | ||
| FLAG-D1 | Ycf48 | 37,267 | 70 | 15 | 54,533,000 |
| P73069 | 342 | ||||
| D1 | 39,695 | 29 | 10 | 26,748,000 | |
| P16033 | 360 | ||||
| Ycf39 | 36,496 | 29 | 10 | 19,328,000 | |
| P74429 | 326 | ||||
| Phb3 | 35,727 | 61 | 18 | 17,022,000 | |
| P72665 | 321 | ||||
| FLAG-D2 | D2 | 39,466 | 21 | 5 | 33,302,000 |
| P09192 | 352 | ||||
| PsbE | 9,442 | 26 | 2 | 18,318,000 | |
| P09190 | 81 | ||||
| PsbY | 4,202 | 21 | 1 | 2,790,100 | |
| P73676 | 39 | ||||
| FtsH2 | 68,496 | 47 | 5 | 563,000 | |
| Q55700 | 627 | ||||
| Phb3 | 35,727 | 12 | 4 | 515,600 | |
| P72665 | 321 | ||||
| FtsH3 | 67,250 | 8 | 4 | 353,700 | |
| P72991 | 616 | ||||
| PsbF | 4,929 | 39 | 1 | 306,100 | |
| P09191 | 44 | ||||
| Slr1470 | 14,902 | 7 | 1 | 182,100 | |
| P74154 | 131 | ||||
| CyanoP | 20,747 | 5 | 1 | 51,900 | |
| P73952 | 188 | ||||
The analysis of proteins precipitated from the preparation was performed using NanoElute UHPLC (Bruker) online coupled to a high-resolution mass spectrometer (Bruker Impact HD).