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. 2022 Apr 26;12(6):jkac096. doi: 10.1093/g3journal/jkac096

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Published by Oxford University Press on behalf of Genetics Society of America 2022. This work is written by US Government employees and is in the public domain in the US.

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Fig. 2. — Expression analysis of PHO2-B and PHO2-C genes in alfalfa. a) RNA-seq reads from flower, stem, nodules, seedpod, leaf, and root tissues from the RegenSY27x genotype were mapped to a modified medsa. XinJiangDaYe.gnm1 assembly (Supplementary Data 1). b) PacBio Iso-seq reads from the same flower, stem, nodules, seedpod, leaf, and root tissues, but at less sequencing depth than the RNA-seq experiment. (c–f) Quantitative RT-PCR expression analysis of PHO2-B and PHO2-C genes from root and leaf tissue grown on low (LP; 10 ppm), optimal (OP; 40 ppm, and high (HP; 60 ppm) phosphate treatments. g) Validation of transcript cleavage by miR399 using an RNA-ligase mediated-rapid amplification of cDNA ends assay (RLM-RACE). The gel shows evidence for haplotypes specific cleavage in the PHO2-B haplotypes but not PHO2-C with the high P treatment. LP is low P treatment, OP is optimal treatment, and HP is high P treatment.